6.4.1 Molecular Biological Properties
 

Lipid-transfer Protein	References
Allergen Nomenclature Mal d 3	(1) Larsen & Lowenstein 1999
Molecular Mass  SDS-PAGE: 13 kDa (2), 9 kDa (1) MALDI-MS: 9058 Da (2)	(1) Pastorello et al. 1999 (2) Sánchez-Monge et al. 1999
Isoelectric Point pI 7.5 (IEF-PAGE)	(1) Pastorello et al. 1999
Amino Acid Sequence N-terminus: aa 1-31 (2): ITXGQVTSSLAPXIGYVRSGGAVPPAXXNGI aa 1-36 (1): ITCGQVTSSLAPCIGYVRSGGAVPPACCNGIRNING	(1) Pastorello et al. 1999 (2) Sánchez-Monge et al. 1999
Posttranslational Modifications Glycosylation: no detection of carbohydrate moieties in SDS-PAGE with periodic acid- Schiff staining (1)	(1) Pastorello et al. 1999
Biological Function Lipid-transfer proteins are involved in plant defense mechanisms and probably participate in formation of extracellular lipophilic substances (cutin, wax) (1)	(1) Sánchez-Monge et al. 1999
Sequence Homology N-terminus of lipid-transfer proteins from almond, peach, and grape: 87%, 86%, and 61% identity to N-terminal sequence (1) N-terminus of pollen allergens from Parietaria judaica (Par j 1.0102) and Brassica rapa: 32% and 23% identity to N-terminal sequence (1)	(1) Sánchez-Monge et al. 1999

 6.4.2 Allergenic Properties
 

Lipid-transfer Protein	References
Frequency of Sensitization IgE-binding to Mal d 3 in 28-100% of patients (1)	(1) see 6.1 Sensitization to Apple Allergens