6.5.1 Molecular Biological Properties
 

Apple Profilin	References
Allergen Nomenclature  Mal d 4 (designated)	(1) Scheurer et al. 1999 (GenBank)
Isoallergens and Variants 3 isoforms (1)	(1) Scheurer et al. 1999 (GenBank)
Molecular Mass  SDS-PAGE: 15 kDa (1), 14 kDa (2)	(1) Vallier et al. 1992 (2) Ebner et al. 1995
Isoelectric Point
Amino Acid Sequence,mRNA, and cDNA Proteins GD4-1* GD4-2* GD4-5* SWISS-PROT:       GenBank: AF129426 AF129427 AF129428 Amino Acids 131 residues 131 residues 131 residues mRNA 396 bp (1) 396 bp (1) 396 bp (1) cDNA       * 77-80% aa identity (BLAST at NBCI)	(1) Scheurer et al. 1999 (GenBank)
Biological Function Profilin family (1)	(1) Scheurer et al. 1999 (GenBank)
Sequence Homology Birch pollen profilin (Bet v 2): aa sequence identity 73-83% (1) Pear profilin (Pyr c 4): aa sequence identity 80-97% (1) Cherry profilin (Pru av 4): aa sequence identy 77-93% (1) Soybean profilin (Gly m 3): 80-86% (1) Latex profilin (Hev b 8): aa sequence identity 81-87% (1)	(1) BLAST at NBCI

 6.5.2 Allergenic Properties
 

Apple Profilin	References
Frequency of Sensitization IgE-binding to profilin in appr. 20% of patients (1)	(1) see 6.1 Sensitization to Apple Allergens