Ara h 5 (peanut allergen)

Allergen Data Collection: Peanut (Arachis hypogaea)
Internet Symposium on Food Allergens 2(2): 87-122 (2000) [http://www.food-allergens.de]

7.5 Properties of Profilin (Ara h 5)

7.5.1 Molecular Biological Properties

Profilin References

Allergen Nomenclature Ara h 5 (1) Larsen & Lowenstein 1999

Molecular Mass
Calculated 14.0 kDa (1) (1) Kleber-Janke et al. 1999

Isoelectric Point Calculated pI 4.6 (1)
(1) Kleber-Janke et al. 1999

Amino Acid Sequence, mRNA, and cDNA

Ara h 5

SWISS-PROT:

GenBank: AF059616

PIR:

Amino acids 131 residues (1)

mRNA 743 bp

cDNA 743 bp (1)

Gene

(1) Kleber-Janke et al. 1999

recombinant Protein
cDNA expression library:
Phage surface display of functional cDNA expression products, selection of cDNA clones on microtiter wells coated with serum IgE from peanut sensitive patients (pJuFo cloning system) (1)
Expression in Escherichia coli:
Subcloning into pDS56/RBSII vector and expression of recombinant Ara h 5 in E.coli M15 cells (1)
(1) Kleber-Janke et al. 1999

Sequence Homology
Profilin from soybean (Gly m 3): aa 83% identity (1) (1) Kleber-Janke et al. 1999

7.5.2 Allergenic Properties

Profilin References

Frequency of Sensitization
IgE-binding to Ara h 5 in 13% of patients (1) (1) see 7.1 Sensitization to Peanut Allergens

Profilin	References
*Frequency of Sensitization* IgE-binding to Ara h 5 in 13% of patients (1)	(1) see 7.1 Sensitization to Peanut Allergens