7.7.1 Molecular Biological Properties
 

Conglutin-homologue Protein	References
Allergen Nomenclature  Ara h 7	(1) Larsen & Lowenstein 1999
Molecular Mass Calculated: 15.8 kDa (1)	(1) Kleber-Janke et al. 1999
Isoelectric Point  Calculated pI 5.6 (1)	(1) Kleber-Janke et al. 1999
Amino Acid Sequence, mRNA, and cDNA Ara h 7   SWISS-PROT:   GenBank: AF091737 PIR:   Amino acids 135 residues (1) mRNA 712 bp cDNA 637 bp (1) Gene	(1) Kleber-Janke et al. 1999
recombinant Protein cDNA expression library: Phage surface display of functional cDNA expression products, selection of cDNA clones on microtiter wells coated with serum IgE from peanut sensitive patients (pJuFo cloning system) (1) Expression in Escherichia coli: Subcloning into pDS56/RBSII vector and expression of recombinant Ara h 7 in E.coli M15 cells (1)	(1) Kleber-Janke et al. 1999
Sequence Homology Ara h 2 peanut allergen: aa 35% identity (1) Ara h 6 peanut allergen: aa 35% identity (1) Conglutin (2S-storage protein) from lupine: aa 39% identity (1)	(1) Kleber-Janke et al. 1999

7.7.2 Allergenic Properties
 

Conglutin-homologue Protein	References
Frequency of Sensitization IgE-binding to Ara h 7 in 43% of patients (1)	(1) see 7.1 Sensitization to Peanut Allergens