Allergen Data Collection: Celery (Apium graveolens)
Internet Symposium on Food Allergens 2(3):145 -67  (2000) [http://www.food-allergens.de]

6.3 Properties of Chlorophyll a/b Binding Protein

6.3.1 Molecular Biological Properties
 
Chlorophyll a/b Binding Protein References
Allergen Nomenclature Api g 3  (proposed name) (1) Scheiner et al. 1997
Molecular Mass  24.8 kDa (not published)
Isoelectric Point  pI 4.87
(not published)
Amino Acid Sequence, mRNA, and cDNA
Api g 3  
GenBank: Z75663
Amino Acids 264
mRNA 963 bp
cDNA  
(1) Hoffmann-Sommergruber et al. (GenBank)
(2) Scheiner et al. 1997
recombinant Protein
Expression in cDNA library:
Api g 3 cloned after selection of IgE-binding clone from celery cDNA expression library (1)
(1) Scheiner et al. 1997
Biological Function / Localization
In chloroplasts associated with the light- harvesting complex (1)
(1) Scheiner et al. 1997
Sequence Homology
80-90% aa sequence identities to chlorophyll a/b binding proteins from white mustard, tomato, soybean, spinach, potato, barley, cucumber, garden pea, maize, rice, and wheat (1)
(1) BLAST at NBCI

 6.3.2 Allergenic Properties
 
Chlorophyll a/b Binding Protein References
Frequency of Sensitization
Probably a minor allergen (IgE-binding in <50% of patients), apart from IgE-screening of the cDNA library the frequency of IgE binding has never been tested in a larger group of patients  (2)
(1) see 6.1 Sensitization to Celery Allergens
(2) Scheiner et al. 1997

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