6.3 Properties of Chlorophyll a/b Binding Protein
6.3.1 Molecular Biological Properties
Chlorophyll a/b Binding Protein | References | ||||||||||
Allergen Nomenclature Api g 3 (proposed name) | (1) Scheiner et al. 1997 | ||||||||||
Molecular Mass 24.8 kDa | (not published) | ||||||||||
Isoelectric
Point pI 4.87
|
(not published) | ||||||||||
Amino Acid Sequence, mRNA,
and cDNA
|
(1) Hoffmann-Sommergruber et al. (GenBank)
(2) Scheiner et al. 1997 |
||||||||||
recombinant Protein
Expression in cDNA library: Api g 3 cloned after selection of IgE-binding clone from celery cDNA expression library (1) |
(1) Scheiner et al. 1997 | ||||||||||
Biological Function / Localization
In chloroplasts associated with the light- harvesting complex (1) |
(1) Scheiner et al. 1997 | ||||||||||
Sequence Homology
80-90% aa sequence identities to chlorophyll a/b binding proteins from white mustard, tomato, soybean, spinach, potato, barley, cucumber, garden pea, maize, rice, and wheat (1) |
(1) BLAST at NBCI |
6.3.2 Allergenic Properties
Chlorophyll a/b Binding Protein | References |
Frequency of Sensitization
Probably a minor allergen (IgE-binding in <50% of patients), apart from IgE-screening of the cDNA library the frequency of IgE binding has never been tested in a larger group of patients (2) |
(1) see 6.1
Sensitization to Celery Allergens
(2) Scheiner et al. 1997 |
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