6.2.1 Molecular Biological Properties
 

Major Hazelnut Allergen	References
Allergen Nomenclature  Cor a 1.0401	(1) Larsen & Lowenstein 2000
Isoallergens and Variants 3 different isoforms by IEF-PAGE (1)	(1) Hirschwehr et al. 1992
Molecular Mass  18 kDa (1), (SDS-PAGE)	(1) Hirschwehr et al. 1992
Isoelectric Point  3 isoforms: pI 5.7, 6.1, and 6.3 (1) pI 5.2-5.8 (2)	(1) Hirschwehr et al. 1992 (2) Caballero et al. 1997
Cor a 1 Isoform Cor a 1.0401 SWISS-PROT:   GenBank: AAD48405 PIR:   Amino Acids 161 aa mRNA   cDNA	(1) Lüttkopf et al. 1999 [GenBank]
Biological Function Bet v 1 family of pathogenisis-related proteins (1)	(1) SWISS-PROT
Sequence Homology of Cor a 1.0401 to: Birch pollen allergen Bet v 1 isoforms: aa 63-78% identity, aa 85% similarity (1) Hazel pollen Cor a 1.01 isoforms: aa 58-60% identity, aa 69-71% similarity (1) Hazel pollen Cor a 1.02 isoform: aa 70% identity (1) Hazel pollen Cor a 1.03 isoform: aa 71% identity (1) Apple allergen Mal d 1 isoforms: aa 55-57% identity, aa 66-69% similarity (1) Celery allergen Api g 1: aa 35% identity, aa 55% similarity (1) Carrot allergen Dau c 1 isoforms: aa 34-35% identity, aa 52% similarity (1)	(1) BLAST at PIR

 6.2.2 Allergenic Properties
 

Major Hazelnut Allergen	References
Frequency of Sensitization IgE-binding to 18 kDa allergen in 100% of patients (1)	(1) see 5.1 Sensitization to Hazel Allergens
Histamine Release Histamine releasing capacity of 18 kDa allergen was demonstrated using blood basophils from 1 hazelnut allergic patient (1)	(1) Hirschwehr et al. 1992
Allergenic Potencies IgE- binding capacities of  18-kDa allergen isoforms: pI 6.3 = pI 6.1 > pI 5.7 (2D-PAGE immunoblot of purified 18-kDa allergen) (1)	(1) Hirschwehr et al. 1992
Cross-reactivity IgE- cross- reactive to rBet v 1 (1)	(1) see 7 Cross-Reactivities