6.3.1 Molecular Biological Properties
Major Pollen Allergen | References | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Allergen Nomenclature Cor a 1.01 - Cor a 1.03 | (1) Larsen & Lowenstein 2000 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Isoallergens and Variants
4 isoallergens or variants by SDS/IEF-PAGE (1) 4 recombinant variants of Cor a 1.01 with aa identities of 96-99% (2) 2 additional isoallergens Cor a 1.02 and Cor a 1.03 (3) |
(1) Rohac et al.
1991
(2) Breiteneder et al. 1993 (3) Hoffmann-Sommergruber et al. 1997 |
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Molecular Mass
17 kDa (1), (SDS-PAGE) |
(1) Rohac et al. 1991 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Isoelectric
Point pI 5.0-5.8 (1)
|
(1) Rohac et al. 1991 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Amino Acid Sequence,mRNA, and
cDNA
|
(1) Breiteneder
et al. 1993
(2) Hoffmann-Sommergruber et al. 1997 |
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recombinant Protein
Expression in Escherichia coli: 4 recombinant isoforms (1, 2) |
(1) Breiteneder
et al. 1993
(2) Schenk et al. 1994 |
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Biological Function
Bet v 1 family of pathogenisis-related proteins (1) |
(1) SWISS-PROT | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sequence Homology
a) of Cor a 1.01 isoforms to: Birch pollen allergen Bet v 1: aa 71-73% identity, 80.5-83% similarity (1) Alder pollen allergen Aln g 1: aa 75.5-76.7% identity, 83.6-85% similarity (1) Hornbeam pollen allergen Car b 1: aa 83.6-89.9% identity, 89.3-95% similarity (1) Apple allergen Mal d 1: aa 54% identity (2) b) of Cor a 1.02 isoform to: Cor a 1.01 isoforms: aa 73-75% identity, aa 85-87% similarity (3) Birch pollen allergen Bet v 1: aa 80-85% identity, 89-93% similarity (3) Apple allergen Mal d 1 isoforms: aa 56-66% identity, aa 72-81% similarity (3) Celery allergen Api g 1: aa 40% identity, aa 61% similarity (3) Carrot allergen Dau c 1 isoforms: aa 40% identity, aa 58% similarity (3) c) of Cor a 1.03 isoform to: Cor a 1.01 isoforms: aa 63-65% identity, aa 74-78% similarity (3) Cor a 1.02 isoform: aa 71% identity (3) Birch pollen allergen Bet v 1: aa 67-73% identity, 78-82% similarity (3) Apple allergen Mal d 1 isoforms: aa 61-71% identity, aa 77-83% similarity (3) Celery allergen Api g 1: aa 39% identity, aa 62% similarity (3) Carrot allergen Dau c 1 isoforms: aa 37% identity, aa 58-60% similarity (3) |
(1) Breiteneder
et al. 1993
(2) Vieths et al. 1996 (3) BLAST at PIR |
6.3.2 Allergenic Properties
Major Pollen Allergen | References | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Frequency of Sensitization
IgE-binding to Cor a 1 in 100% of patients (1) |
(1) see 5.1 Sensitization to Hazel Allergens | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Allergenic Potencies
Differential IgE reactivity to 4 rCor a 1 isoforms by samples of 5 hazelpollen allergic patients (immunoblot) (1, 2) |
(1) Breiteneder
et al. 1993
(2) Schenk et al. 1994 |
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Cross-reactivity
IgE- cross- reactive to rBet v 1 (1) |
(1) see 7 Cross-Reactivities | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
T-Cell Proliferation / Epitopes
Stimulation of Bet v 1 specific T-cell clones with natural Cor a 1 (1) Stimulation of Bet v1 specific T-cell clones from birch pollen and hazelpollen allergic patients with natural and recombinant Cor a 1 isoforms (2):
Stimulation of Bet v1 specific T-cell clones from birch pollen and hazelpollen allergic patients with synthetic peptides according to internal sequences of rCor a 1 isoforms and rBet v 1 (2):
Amino acids critical for T-cell proliferation with synthetic
peptides (2):
|
(1) Ebner et al.
1993
(2) Schenk et al. 1994 |
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