6.2.1 Molecular Biological Properties
| Soybean Hydrophobic Protein | References |
| Allergen Nomenclature
a) Gly m 1.0101 (Gly mIA, S2) b) Gly m 1.0102 (Gly mIB, S1) |
(1) Larsen & Lowenstein 1998 |
| Molecular Mass a) 7.5 kDa b) 7.0 kDa | (1) Gonzalez et al. 1992 |
|
Isoelectric
Point a) pI 6.8 b) pI 6.1-6.2
|
(1) Gonzalez et al. 1992 |
| Amino Acid Sequence
PIR: A29385 a) N-terminal sequence of 42 aa (2) matching the sequence of hydrophobic seed protein, 80 aa (1) b) lacking N-terminal tripeptide (2) |
(1) Odani et al. 1987
(2) Gonzalez et al. 1995 |
| 3D-Structure
X-ray studies (1) |
(1) Baud et al. 1993 |
| Posttranslational Modifications
Disulfide bonds 4 disulfide bonds (1) Glycosylation: carbohydrate detection (immunoblot) (2) |
(1) Odani et al. 1987
(2) Gonzalez et al. 1992 |
| Genetic Variants
b) 2 isoforms by IEF-PAGE (1) |
(1) Gonzalez et al. 1992 |
6.2.2 Allergenic Properties
| Soybean Hydrophobic Protein | References |
| Frequency of Sensitization
IgE-binding to Gly m 1 in 95% of patients (1) |
(1) see 6.1 Sensitization to Soybean Allergens |
| Allergenic Potencies
IgE-binding inhibition of total soybean extract: 72% (a) and 76% (b) (EAST) Concentrations of 50%-inhibition: (a) lower concentrations as (b) (1) (a) Gly m 1.0101 (b) Gly m 1.0102 |
(1) Gonzalez et al. 1992 |