Gly m 3 (Soybean Profilin)

6.4 Properties of Soybean Profilin (Gly m 3)

6.4.1 Molecular Biological Properties

Soybean Profilin References

Allergen Nomenclature Gly m 3 (1) Larsen & Lowenstein 1998

Molecular Mass 14 kDa (1) SWISS-PROT

Isoelectric Point

Amino Acid Sequence, mRNA, and cDNA

Proteins Profilin 1* Profilin 2*

SWISS-PROT: O65809 O65810

GenBank: AJ223982 AJ223981

Amino Acids 131 (1) 131 (1)

mRNA precursor 396 bp (1) 396 bp (1)

cDNA precursor

* 97.7% identity (1) Rihs et al. 1999

recombinant Profilin
Expression in Escherichia coli:
rGly m 3 (Profilin 1) (1)
fragments: aa 1-65, 38-88, 50-131 (1) (1) Rihs et al. 1999

Genetic Variants
2 isoforms (1) (1) Rihs et al. 1999

Biological Function
Binds to actin (1:1), affects structure of the cytoskeleton
profilin family (1) (1) SWISS-PROT

6.4.2 Allergenic Properties

Soybean Profilin References

Frequency of Sensitization
IgE-binding to profilin in 69% of patients (1) (1) see 6.1 Sensitization to Soybean Allergens

B-Cell Epitopes
IgE binding to rGly m 3, inactive recombinant fragments (aa 1-65, 38-88, 50-131) indicating conformational epitopes (1)
(a) SDS-PAGE / immunoblot
(b) EAST / EAST inhibition
(1) Rihs et al. 1999

Soybean Profilin	References
*Frequency of Sensitization* IgE-binding to profilin in 69% of patients (1)	(1) see 6.1 Sensitization to Soybean Allergens
*B-Cell Epitopes* IgE binding to rGly m 3, inactive recombinant fragments (aa 1-65, 38-88, 50-131) indicating conformational epitopes (1) (a) SDS-PAGE / immunoblot (b) EAST / EAST inhibition	(1) Rihs et al. 1999