5.2 Properties of Actinidin

5.2.1 Molecular Biological Properties
 

Actinidin	References
Allergen Nomenclature  Act c 1	(1) Larsen & Lowenstein 1998
Isoallergens and Variants Sequence variation in 2 kiwi varieties (1)	(1) Naylor et al. 1989
Molecular Mass Mr 30 kDa	(1) Pastorello et al. 1998
Isoelectric Point  pI 3.5 (1, 2)	(1) Fahlbusch et al. 1998 (2) Pastorello et al. 1998
Amino Acid Sequence, mRNA, and cDNA Act c 1         SWISS-PROT: P00785       GenBank: X16466 X13013 X13139 X57551 PIR: TAGB S02728 S02729   Amino acids 254 aa  (1) 1-15 (5) P* (6)   mRNA 1370 bp  ( 3) 1138 bp (2) 809 bp (2)   cDNA       634 bp (4) * 5 partial sequences from tryptic digest (6)	(1) Carne & Moore 1978 (2) Praekelt et al. 1988 (3) Podivinsky et al. 1989 (4) Keeling et al.1990 (5) Fahlbusch et al. 1998 (6) Pastorello et al. 1998
recombinant Actinidin expression in tabacco (1)	(1) Paul et al. 1995
3D-Structure X-ray studies (1) complex with inhibitor (2)	(1) Baker 1980 (2) Varughese 1992
Posttranslational Modifications Disulfide bonds: 3 disulfide bonds  (1)  Glycosylation: No glycosylation by lectin binding (2) No glycosylation by periodic acid-Schiff stain (3)	(1) SWISS-PROT:  P00785 (2) Fahlbusch et al. 1998 (3) Pastorello et al. 1998
Biological Function enzyme: thiol-protease, EC 3.4.22.14 specificity close to papain (1)	(1) Baker et al. 1980
Sequence Homology Actinidin has a similar structure to Der p 1, the major allergen of house dust mite (Dermatophagoides pteronyssinus) (1)	(1) Tropham et al. 1994

5.2.2 Allergenic Properties
 

Actinidin	References
Frequency of Sensitization IgE-binding to Act c 1 in 19 to 100% of patients (1)	(1) see 5.1 Sensitization to Kiwi Fruit Allergens