6.7.1 Molecular Biological Properties
 

beta-Conglycinin	References
Allergen Nomenclature none
Molecular Mass  140-180 kDa 3 subunits (SEC, SDS-PAGE): alpha 76 kDa (2), 70 kDa (3), 57 kDa (1) alpha' 72 kDa (2), 57 kDa (1) beta 53 kDa (1), 42 kDa (1)	(1) Thanh & Shibasaki 1977 (2) Shattuck-Eidens & Beachy 1985 (3) Ogawa et al. 1995
Isoelectric Point pI alpha 4.90, alpha' 5.18, beta 5.66-6.00 (gel electrofocusing)	(1) Thanh & Shibasaki 1977
Amino Acid Sequence, mRNA, and cDNA beta- conglycinin (subunits) alpha alpha' beta SWISS-PROT: P13916 P11827 P25974 GenBank: X17698 M13759 S44893 Amino Acids 543 (1) 577 (1) 414 (3) mRNA precursor 1955 bp (4)     cDNA precursor   3636 bp (2) 3840 bp (3)	(1) Schuler et al. 1982 (2) Doyle et al. 1986 (3) Harada et al. 1989 (4) Sebastiani et al. 1990
recombinant Protein Expression in Arabidopsis thaliana: alpha' and beta subunits (3) Expression in Escherichia coli: non-glycosylated subunits and deletion mutants of alpha and alpha' (4) Expression in petunia and tobacco plants: alpha' and beta subunits (1) SP6-derived expression plasmids: non-glycosylated alpha and beta subunits (2)	(1) Chen et al. 1989 (2) Lelievre et al. 1992 (3) Hirai et al. 1995 (4) Maruyama et al. 1998
Posttranslational Modifications Glycosylation: carbohydrates: alpha and alpha' subunits contain 4 mol Man, 12 mol GlcN and beta subunit 6 mol Man, 2 mol GlcN(1) alpha and alpha': 2 carbohydrate moieties, beta chain: 1 carbohydrate moiety (1)	(1) Thanh & Shibasaki 1977
Biological Function Seed storage protein

 6.7.2 Allergenic Properties
 

beta-Conglycinin	References
Frequency of Sensitization IgE-binding to 7S fraction (1) to alpha, alpha' and beta- subunits (2) to alpha-subunit in 23% of patients (3) no cross- reactivity to alpha'- and beta- subunits (3)	(1) see 6.1 Sensitization to Soybean Allergens (2) Burks et al. 1988 (3) Ogawa et al. 1995
B-Cell Epitopes IgE-binding region on alpha- subunit: aa 232-383 (enzymatic digestion) (1) (a) SDS-PAGE / immunoblot	(3) Ogawa et al. 1995