6.2.1 Molecular Biological Properties
 

Soybean Hydrophobic Protein	References
Allergen Nomenclature a) Gly m 1.0101 (Gly mIA, S2) b) Gly m 1.0102 (Gly mIB, S1)	(1) Larsen & Lowenstein 1998
Isoallergens and Genetic Variants b) 2 isoforms by IEF-PAGE (1)	(1) Gonzalez et al. 1992
Molecular Mass a) 7.5 kDa b) 7.0 kDa	(1) Gonzalez et al. 1992
Isoelectric Point a) pI 6.8 b) pI 6.1-6.2	(1) Gonzalez et al. 1992
Amino Acid Sequence PIR: A29385 a) N-terminal sequence of 42 aa (2) matching the sequence of hydrophobic seed protein, 80 aa (1) b) lacking N-terminal tripeptide (2)	(1) Odani et al. 1987 (2) Gonzalez et al. 1995
3D-Structure X-ray studies (1)	(1) Baud et al. 1993
Posttranslational Modifications Disulfide bonds 4 disulfide bonds (1) Glycosylation: carbohydrate detection (immunoblot) (2)	(1) Odani et al. 1987 (2) Gonzalez et al. 1992
Localization Predominantly located on seed surface; amounts vary among soybean cultivars, higher on dull-seeded phenotypes (SDS-PAGE, micro-sequencing) (1) Pattern of gene expression shows that Gly m 1 is synthesized in endocarp of inner ovary wall and is deposited on seed surface during development (in situ hybridization, RNA analysis) (1)	(1) Gijzen et al. 1999

 6.2.2 Allergenic Properties
 

Soybean Hydrophobic Protein	References
Frequency of Sensitization IgE-binding to Gly m 1 in 95% of patients (1)	(1) see 6.1 Sensitization to Soybean Allergens
Allergenic Potencies IgE-binding inhibition of total soybean extract: 72% (a) and  76% (b)  (EAST) Concentrations of 50%-inhibition: (a) lower concentrations as (b) (1) (a) Gly m 1.0101 (b) Gly m 1.0102	(1) Gonzalez et al. 1992