6.4.1 Molecular Biological Properties
Soybean Profilin | References | ||||||||||||||||||
Allergen Nomenclature Gly m 3 | (1) Larsen & Lowenstein 1998 | ||||||||||||||||||
Isoallergens and Variants
2 isoforms differing in 3 aa substitutions (1) |
(1) Rihs et al. 1999 | ||||||||||||||||||
Molecular Mass 14 kDa (SDS-PAGE) | (1) Rihs et al. 1999 | ||||||||||||||||||
Isoelectric
Point pI 4.4 (2D-PAGE)
|
(1) Rihs et al. 1999 | ||||||||||||||||||
Amino Acid Sequence, mRNA,
and cDNA
|
(1) Rihs et al. 1999 | ||||||||||||||||||
recombinant Profilin
Expression in Escherichia coli: rGly m 3 (Profilin 1) (1) fragments: aa 1-65, 38-88, 50-131 (1) |
(1) Rihs et al. 1999 | ||||||||||||||||||
Biological Function
Binds to actin (1:1), affects structure of the cytoskeleton profilin family (1) |
(1) SWISS-PROT | ||||||||||||||||||
Sequence Homology
11 plant profilins: aa sequence identies 69% to 88% (1) Bet v 2 from birch pollen: aa sequence identity 73% (1) |
(1) Rihs et al. 1999 |
6.4.2 Allergenic Properties
Soybean Profilin | References |
Frequency of Sensitization
IgE-binding to profilin in 69% of patients (1) |
(1) see 6.1 Sensitization to Soybean Allergens |
B-Cell Epitopes
IgE binding to rGly m 3, inactive recombinant fragments (aa 1-65, 38-88, 50-131) indicating conformational epitopes (1) (a) SDS-PAGE / immunoblot
|
(1) Rihs et al. 1999 |
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