Allergen Data Collection - Update: Soybean (Glycine max)
Internet Symposium on Food Allergens 2(Suppl.3): 1-35 (2000) [http://www.food-allergens.de]
6.4 Properties of Soybean Profilin (Gly m 3)

6.4.1 Molecular Biological Properties
 
Soybean Profilin References
Allergen Nomenclature  Gly m 3 (1) Larsen & Lowenstein 1998
Isoallergens and Variants
2 isoforms differing in 3 aa substitutions (1)
(1) Rihs et al. 1999
Molecular Mass 14 kDa (SDS-PAGE) (1) Rihs et al. 1999
Isoelectric Point pI 4.4 (2D-PAGE)
(1) Rihs et al. 1999
Amino Acid Sequence, mRNA, and cDNA
Proteins GmPRO 1* GmPRO 2*
SWISS-PROT: O65809 O65810
GenBank: AJ223982 AJ223981
Amino Acids 131 (1) 131 (1)
mRNA precursor 394 bp (1)  
cDNA precursor    
* 97.7% identity
(1) Rihs et al. 1999
recombinant Profilin
Expression in Escherichia coli:
rGly m 3  (Profilin 1) (1)
fragments: aa 1-65, 38-88, 50-131 (1)
(1) Rihs et al. 1999
Biological Function
Binds to actin (1:1), affects structure of the cytoskeleton
profilin family (1)
(1) SWISS-PROT
Sequence Homology
11 plant profilins: aa sequence identies 69% to 88% (1)
Bet v 2 from birch pollen: aa sequence identity 73% (1)
(1) Rihs et al. 1999

 6.4.2 Allergenic Properties
 
Soybean Profilin References
Frequency of Sensitization
IgE-binding to profilin in 69% of patients (1)
(1) see 6.1 Sensitization to Soybean Allergens
B-Cell Epitopes
IgE binding to rGly m 3,  inactive recombinant fragments (aa 1-65, 38-88, 50-131) indicating conformational epitopes (1)

(a) SDS-PAGE / immunoblot
(b) EAST / EAST  inhibition

(1) Rihs et al. 1999

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