6.8.1 Molecular Biological Properties
 

Kunitz-trypsin Inhibitor (KTI)	References
Allergen Nomenclature none
Molecular Mass  20 kDa (SDS-PAGE)	(1) Baur et al. 1996
Isoelectric Point pI ~4.5 (2D-PAGE)	(1) Baur et al. 1996
Amino Acid Sequence,mRNA, and cDNA Kunitz- trypsin- inhibitor A B C SWISS-PROT: P01070 P01071 P01070 PIR: TISY TISYB TISYC GenBank: S45092 Amino Acids (1) 181 181 181 mRNA precursor 1259 bp (2) cDNA precursor	(1) Kim et al. 1985 (2) Jofuku et al. 1989
recombinant Protein Expression in tobacco plants: all 3 variants  (1)	(1) Jofuku & Goldberg 1989
3D-Structure X-ray studies of complex with trypsin (1, 2)	(1) Sweet et al. 1974 (2) Song & Suh 1998
Posttranslational Modifications Disulfide bonds 2 disulfide bonds: 39-86, 136-145 (1)	(1) Brown et al. 1966
Genetic Variants  3 variants (1)	(1) Kim et al. 1985
Biological Function inhibition of trypsin active site: Arg-63 (KTI A) (1)	(1) Ozawa & Laskowski 1966
Sequence Homology Lol p 11, a rye grass pollen allergen: 32% homology (1)	(1) van Ree et al. 1995

 6.8.2 Allergenic Properties
 

Kunitz-trypsin Inhibitor (KTI)	References
Frequency of Sensitization IgE-binding to Kunitz-trypsin inhibitor in 86% of patients (1)	(1) see 6.1 Sensitization to Soybean Allergens