Recombinant Allergen |
Tested by |
References |
|
Celery (Apium graveolens) |
Api g 1*
Expressed in
E.coli
Fusion peptide
-- |
immunoblot:
sera of 10 celery-allergic patients with positive SPT and
symptoms after ingestion of celery (1)
sera of 24 celery-allergic patients
of Central Europe with clinical history and positive SPT to extract (5)
sera of 12 celery-allergic patients
of Mediterranean area with positive case history and positive SPT to crude
celery (5)
sera of 22 celery-allergic patients proved by DBPCFC and
SPT (6)
immunoblot inhibition:
serum of 1 patient with multiple inhalant and food allergies
(4)
pooled serum of 12 celery-allergic patients with positive
SPT and symptoms after ingestion of celery (1)
sera of 2 celery-allergic patients proved by DBPCFC and SPT
(6)
RAST:
sera of 24 celery-allergic patients of Central Europe with
clinical history and positive SPT to extract (5)
sera of 12 celery-allergic patients of Mediterranean area
with positive case history and positive SPT to crude celery (5)
EAST:
-
sera of 30 patients with adverse reactions after ingestion
of celery tuber (2)
-
sera of 9 patients with celery-related symptoms after ingestion
of celery and IgE against Api g 1 and / or CCD and serum of 1 patient with
multiple inhalant and food allergies (4)
-
sera of 22 celery-allergic patients proved by DBPCFC and
SPT (6)
EAST inhibition:
-
serum of 1 celery-allergic patient with positive DBPCFC (3)
-
sera of 9 patients with celery-related symptoms after ingestion
of celery and IgE against Api g 1 and / or CCD and serum of 1 patient with
multiple inhalant and food allergies (4)
-
sera of 3 patients with celery-related symptoms after ingestion
of celery and IgE against Api g 1 and / or CCD (4)
-
sera of 3 celery-allergic patients proved with DBPCFC and
SPT (6)
mediator release:
-
serum of 1 patient with celery-related symptoms after ingestion
of celery and IgE against celery tuber (4)
SPT:
sera of 24 celery-allergic patients of Central Europe with
clinical history and positive SPT to extract (5)
sera of 12 celery-allergic patients of Mediterranean area
with positive case history and positive SPT to crude celery but not to
extract (5)
|
(1) Breiteneder et al. 1995
(2) Jankiewicz et al. 1998
(3) Scheurer et al. 2000b
(4) Fötisch et al. 1999
(5) Hoffmann-Sommergruber et al. 1999a
(6) Lüttkopf et al. 2000a |
Api g 1.0201
Expressed in
E.coli
Fusion peptide
his6-tag |
immunoblot:
-
sera of 10 celery-allergic patients
immunoblot inhibition:
-
pooled serum of 4 celery-allergic patients
|
Hoffmann-Sommergruber et al. 2000 |
Api g 2
Expressed in
E.coli
Fusion peptide
-- |
-
(reported, data not shown) test with sera from celery-allergic
and birch pollen-allergic patients; test and number of sera not mentioned
|
Scheiner et al. 1997 |
Api g 4*
Expressed in
E.coli
Fusion peptide
-- |
immunoblot:
-
sera of 17 patients: 13/17 sera of patients with positive
DBPCFC to celery, 4/17 sera of patients with clinical symptoms to celery
and positive allergosorbent test to birch profilin (1)
-
sera of 22 celery-allergic patients proved by DBPCFC and
SPT (3)
immunoblot inhibition:
-
sera of 9 patients: 3/9 sera of patients with positive DBPCFC
to celery, 6/9 sera of patients with clinical symptoms to celery and positive
allergosorbent test to birch profilin (1)
-
sera of 2 celery-allergic patients with positive allergosorbent
test to birch pollen profilin (1)
-
pooled serum of 9 celery-allergic patients (1)
-
sera of 2 celery-allergic patients proved by DBPCFC and SPT
(3)
EAST:
-
sera of 22 celery-allergic patients proved by DBPCFC and
SPT (3)
-
(reported, data not shown) sera of 10 patients with a history
to celery (2)
-
(reported, data not shown) sera of 49 allergic subjects with
positive EAST to recombinant birch profilin (2)
EAST inhibition:
-
1 individual serum and pooled serum of 2 celery-allergic
patients with IgE against birch profilin (1)
-
sera of 22 celery-allergic patients proved by
-
DBPCFC and SPT (3)
mediator release:
-
serum of 1 celery-allergic patient with positive allergosorbent
test to birch pollen profilin (1)
-
(reported, data not shown) (2)
|
(1) Scheurer et al. 2000b
(2) Scheurer et al. 2000a
(3) Lüttkopf et al. 2000a |
|
Peanut (Arachis hypogaea) |
Ara h 1*
Expressed in
E.coli (1, 2, 3)
yeast: Pichia pastoris (4)
Fusion peptide
37 amino acids of beta-galactosidase
(1);
his6-tag (3);
-- (4) |
immunoblot:
-
sera of 18 patients with peanut hypersensitivity confirmed
by positive SPT to peanut and positive DBPCFC or clinical history of peanut
anaphylaxis (1)
-
pooled serum of patients with peanut hypersensitivity confirmed
by positive SPT to peanut and positive DBPCFC or clinical history of peanut
anaphylaxis (1)
-
sera of 11 and of 3 peanut-hypersensitive patients (2)
-
sera of 40 patients with clinical symptoms after peanut ingestion,
positive SPT, RAST class to peanut > 3 (3)
-
sera of 5 peanut-allergic patients (4)
|
(1) Burks et al. 1995b
(2) Burks et al. 1995a
(3) Kleber-Janke et al. 1999
(4) Maid 1998 |
Ara h 2*
Expressed in
E.coli
Fusion peptide
his6-tag (1, 2, 3) |
immunoblot:
-
sera of 40 patients with clinical symptoms after peanut ingestion,
positive SPT, RAST class to peanut > 3 (1)
-
(reported, data not shown) 16 peanut-sensitive patients (2,
3)
|
(1) Kleber-Janke et al. 1999
(2) King et al. 1999
(3) Burks et al. 1999 |
Ara h 3*
Expressed in
E.coli
Fusion peptide
his6-tag |
immunoblot:
-
sera of 18 peanut-allergic patients with positive SPT and
either positive DBPCFC or history of anaphylaxis
-
pooled serum of peanut-allergic patients with positive SPT
and either positive DBPCFC or history of anaphylaxis
|
Rabjohn et al. 1999 |
Ara h 4*
Expressed in
E.coli
Fusion peptide
his6-tag |
immunoblot:
-
sera of 40 patients with clinical symptoms after peanut ingestion,
positive SPT, RAST class to peanut > 3
-
pooled serum of 34 peanut-sensitised patients
|
Kleber-Janke et al. 1999 |
Ara h 5*
Expressed in
E.coli
Fusion peptide
his6-tag |
immunoblot:
-
sera of 40 patients with clinical symptoms after peanut ingestion,
positive SPT, RAST class to peanut > 3
-
pooled serum of 34 peanut-sensitised patients
|
Kleber-Janke et al. 1999 |
Ara h 6*
Expressed in
E.coli
Fusion peptide
his6-tag |
immunoblot:
-
sera of 40 patients with clinical symptoms after peanut ingestion,
positive SPT, RAST class to peanut > 3
-
pooled serum of 34 peanut-sensitised patients
|
Kleber-Janke et al. 1999 |
Ara h 7*
Expressed in
E.coli
Fusion peptide
his6-tag |
immunoblot:
-
sera of 40 patients with clinical symptoms after peanut ingestion,
positive SPT, RAST class to peanut > 3
-
pooled serum of 34 peanut-sensitised patients
|
Kleber-Janke et al. 1999 |
|
Milk (Bos domesticus) |
Bos d 5*
allergen here: met-beta-lactoglobulin (lacking the first
ten amino acids of the mature protein)
Expressed in
E.coli
Fusion peptide
-- |
ELISA:
-
sera of 5 patients allergic to beta-lactoglobulin
|
Chatel et al. 1996 |
|
Rapeseed (Brassica napus) |
Bra n ?
note: in reference denominated BnI
Expressed in
yeast: Pichia pastoris |
immunoblot:
-
(reported, not shown) sera of mustard-allergic patients
ELISA:
-
(reported, data not shown) sera of mustard-allergic patients
|
Villalba et al. 2000 |
|
Crab (Charybdis feriatus) |
Cha f 1
Expressed in
E.coli
Fusion peptide
glutathione S transferase |
immunoblot:
-
10 individual sera and pooled serum of patients with type
I hypersensitivity upon ingestion of crab and IgE to shrimp, lobster and
crab
immunoblot inhibition:
-
pooled serum of patients with type I hypersensitivity upon
ingestion of crab and IgE to shrimp, lobster and crab
|
Leung et al. 1998a |
|
Scallop (Chamys nobilis) |
Cham n ?
Expressed in
E.coli |
immunoblot and inhibition studies:
-
(reported, data not shown) sera of shellfish-allergic subjects
|
Leung et al. 2000 |
|
Hazelnut (Corylus avellana) |
Cor a 1.0401*
Expressed in
E.coli
Fusion peptide
-- |
immunoblot:
-
(reported, data not shown) sera of 34 hazelnut-allergic patients
with positive DBPCFC (2)
EAST:
-
sera of 13 food-allergic patients with positive DBPCFC to
hazelnut (1)
-
(data reported, not shown) sera of 43 food-allergic patients
with positive DBPCFC to hazelnut (3)
inhibition assay:
-
(reported, data not shown) (1)
mediator release:
-
(reported, data not shown ) sera of 6 patients with positive
DBPCFC to hazelnut (1)
|
(1) Vieths et al. 2000
(2) Lüttkopf et al. 1999
(3) Lüttkopf et al. 2000b |
|
Carp (Cyprinus carpio) |
Parvalbumin
Expressed in
E.coli
Fusion peptide
beta-galactosidase |
immunoblot:
-
sera of 10 fish-allergic patients (1)
IgE-binding assay:
-
(reported, data not shown) sera of fish-allergic patients;
test and number of sera not mentioned (2)
|
(1) Bugajska-Schretter et al. 1999
(2) Bugajska-Schretter et al. 1998 |
|
Carrot (Daucis carota) |
Dau c 1
Expressed in
E.coli
Fusion peptide
his6-tag |
immunoblot:
-
sera of 6 carrot-allergic patients with positive SPT to fresh
carrot and positive case history
immunoblot inhibition:
-
pooled serum of 6 carrot-allergic patients with positive
SPT to fresh carrot and positive case history
|
Hoffmann-Sommergruber et al. 1999b |
Dau c LTP
Expressed in
yeast: Pichia pastoris
Fusion peptide
-- |
RAST inhibition:
-
serum of 1 pollen- and apple-allergic patient with sensitisation
to LTP
|
Asero et al. 2000b |
|
Soybean (Glycine maxima) |
Gly m 1*
Expressed in
E.coli
Fusion peptide
his6-tag |
IgE-binding test:
-
(reported, data not shown) test and sera not mentioned
|
Beardslee et al. 1999 |
Gly m 3*
Expressed in
E.coli
Fusion peptide
-- |
immunoblot:
-
8 individual sera and a pooled serum of 7 subjects with adverse
reactions to food and IgE against soybean proteins
immunoblot inhibition:
-
3 individual sera and pooled serum of patients with adverse
reactions to food and IgE against soybean proteins
EAST inhibition:
-
pooled serum of subjects with adverse reactions to food and
IgE against soybean proteins
|
Rihs et al. 1999 |
Gly m 3*
Expressed in
E.coli
Fusion peptide
maltose-binding protein |
immunoblot:
-
8 individual sera and a pooled serum of 7 subjects with adverse
reactions to food and IgE against soybean proteins
EAST:
-
sera of 13 subjects with adverse reactions to food and IgE
against soybean proteins
|
Rihs et al. 1999 |
Gly m ?
2 glycinin chains
Expressed in
E.coli
Fusion peptide
his6-tag |
IgE-binding test:
-
(reported, data not shown) test and sera not mentioned
|
Beardslee et al. 1999 |
|
Abalone (Haliotis diversicolor) |
Hal d ?
Expressed in
E.coli |
immunoblot and inhibition studies:
-
(reported, data not shown) sera of shellfish-allergic subjects
|
Leung et al. 2000 |
|
Lobster (Homarus americanus) |
Hom a 1
Expressed in
E.coli
Fusion peptide
glutathione S transferase |
immunoblot:
-
sera of 10 patients with type I hypersensitivity upon ingestion
of crab and IgE against lobster, shrimp and crab (1)
-
pooled serum of 10 patients with crustacean allergy (2)
immunoblot inhibition:
-
pooled serum of 10 patients with crustacean allergy (2)
|
(1) Leung et al. 1998a
(2) Leung et al. 1998b |
|
Walnut (Juglans regia) |
Jug r 1*
Expressed in
E.coli
Fusion peptide
glutathione S transferase |
immunoblot:
-
sera of 16 patients with allergic reactions to walnuts and
FEIA class 2 and higher to walnut extract
FEIA inhibition:
-
sera of 16 patients with allergic reactions to walnuts and
FEIA class 2 and higher to walnut extract
|
Teuber et al. 1998 |
Jug r 2*
Expressed in
E.coli
Fusion peptide
glutathione S transferase |
immunoblot:
-
sera of 15 patients with systemic reactions to walnuts
immunoblot inhibition:
-
sera of 4 patients with systemic reactions to walnuts and
positive IgE immunoblot to rJug r 2
|
Teuber et al. 1999 |
Jug r ?
Expressed in
E.coli
Fusion peptide
not specified |
IgE-binding assay:
-
(reported, data not shown) sera of 12 walnut-allergic patients,
test not mentioned
|
Teuber et al. 1996 |
|
Apple (Malus domesticus) |
Mal d 1*
Expressed in
E.coli
Fusion peptide
-- |
immunoblot:
-
sera of 9 patients with allergy to apple and birch pollen
according to positive case history and RAST class 2.5 and higher (1)
-
sera of 8 birch pollen-allergic patients with symptoms after
ingestion of fresh apples, positive SPT to apple extract and positive EAST
to natural Mal d 1 class 2 and higher (2)
immunoblot inhibition:
-
pooled serum of 5 patients with allergy to apple and birch
pollen according to positive case history and RAST class 2.5 and higher
(1)
EAST:
-
sera of 13 birch pollen-allergic patients with symptoms after
ingestion of fresh apples, positive SPT to apple extract and positive EAST
to natural Mal d 1 class 2 and higher (2)
mediator release: (2, 3) |
(1) Vanek-Krebitz et
al. 1995
(2) Son et al. 1999
(3) Scheurer et al. 1999 |
|
Shrimp (Metapenaeus ensis) |
Met e 1*
Expressed in
E.coli
Fusion peptide
glutathione S transferase |
immunoblot:
-
pooled serum of shrimp-allergic patients (1)
-
sera of 10 patients with type I hypersensitivity upon ingestion
of crab and IgE to shrimp, lobster and crab (2)
immunoblot inhibition:
-
(reported, data not shown) pooled serum of 9 patients with
anaphylactic reactions after ingestion of shrimp (3)
|
(1) Leung et al. 1994
(2) Leung et al. 1998a
(3) Leung et al. 1996 |
|
Lobster (Panulirus stimpsoni) |
Pan s 1
Expressed in
E.coli
Fusion peptide
glutathione S transferase |
immunoblot:
-
pooled serum of 10 patients with crustacean allergy (1)
-
sera of 10 patients with type I hypersensitivity upon ingestion
of crab and IgE to shrimp, lobster and crab (2)
immunoblot inhibition:
-
pooled serum of 10 patients with crustacean allergy (1)
|
(1) Leung et al. 1998b
(2) Leung et al. 1998a |
|
Shrimp (Penaeus aztecus) |
Pen a 1*
Expressed in
E.coli
Fusion peptide
-- |
immunoblot:
-
pooled serum of shrimp-allergic patients
|
Reese et al. 1997 |
|
Mussel (Perna viridis) |
Pern v ?
Expressed in
E.coli |
immunoblot and inhibition studies:
-
(reported, data not shown) sera of shellfish-allergic subjects
|
Leung et al. 2000 |
|
Avocado (Persea americana) |
Pers a 1*
note: in reference denominated Prs a 1
Expressed in
yeast: Pichia pastoris
Fusion peptide
-- |
immunoblot:
-
pooled serum of patients with positive case history, positive
SPT, RAST class 4 and higher and type I allergy to latex and some patients
with additional symptoms after ingestion of avocado
immunoblot inhibition:
-
pooled serum of patients with positive case history, positive
SPT, RAST class 4 and higher and type I allergy to latex and some patients
with additional symptoms after ingestion of avocado
|
Sowka et al. 1998 |
|
Cherry (Prunus avium) |
Pru av 1*
note: in references denominated Pru a 1
Expressed in
E.coli
Fusion peptide
-- |
immunoblot:
-
sera of 7 birch pollen-allergic patients with symptoms after
ingestion of fresh cherries, positive SPT to cherry extract and birch pollen
extract and EAST class 2 and higher (2)
immunoblot inhibition:
-
pooled serum of 4 birch pollen-allergic patients with symptoms
after ingestion of fresh cherries and positive SPT to cherry extract (1)
-
pooled serum of 4 birch pollen-allergic patients with symptoms
after ingestion of fresh cherries, positive SPT to cherry extract and birch
pollen extract and EAST class 2 and higher (2)
EAST:
-
sera of 19 birch pollen-allergic patients with clinical symptoms
after ingestion of cherry and positive SPT to cherry extract (1)
-
sera of 7 birch pollen-allergic patients with symptoms after
ingestion of fresh cherries, positive SPT to cherry extract and birch pollen
extract and EAST class 2 and higher (2)
mediator release:
-
sera of 3 birch pollen-allergic patients with clinical symptoms
after ingestion of fresh cherries (1)
-
mediator release (2)
|
(1) Scheurer et al. 1997
(2) Scheurer et al. 1999 |
Pru av 1*
note: in references denominated Pru a 1
Expressed in
E.coli
Fusion peptide
his 10-tag |
immunoblot:
-
sera of 9 birch pollen-allergic patients with clinical symptoms
after ingestion of fresh cherries and positive SPT to cherry extract (1)
-
pooled serum of 4 birch pollen-allergic patients with symptoms
after ingestion of fresh cherries, positive SPT to cherry extract and birch
pollen extract and EAST class 2 and higher (2)
immunoblot inhibition:
-
pooled serum of 4 birch pollen-allergic patients with symptoms
after ingestion of fresh cherries, positive SPT to cherry extract and birch
pollen extract and EAST class 2 and higher (1, 2)
|
(1) Scheurer et al. 1997
(2) Scheurer et al. 1999 |
Pru av 4*
note: in reference denominated Pru a 4
Expressed in
E.coli
Fusion peptide
-- |
immunoblot:
-
(reported, data not shown) number and quality of sera not
mentioned
EAST:
-
(reported, data not shown) sera of 13 patients with symptoms
after ingestion of cherry
-
(reported, data not shown) sera of 49 subjects with positive
EAST to recombinant birch profilin
|
Scheurer et al. 2000a |
|
Pear (Pyrus communis) |
Pyr c 1*
Expressed in
E.coli
Fusion peptide
-- |
immunoblot:
-
sera of 23 patients with pollen-related allergy to pear and
EAST to pear > 0.7 U/ml (1, 2)
immunoblot inhibition:
-
serum of 1 patient with pollen-related allergy to pear (1)
-
sera of 2 subjects with EAST to pear class 2 or 3, respectively
(2)
EAST:
-
sera of 36 or 61 patients with pollen-related allergy to
pear (1, 2, 4)
EAST inhibition:
-
sera of 8 subjects with or without symptoms after ingestion
of pear but positive EAST to pear class 2 and higher (2)
mediator release:
-
sera of 5 patients with oral allergy syndrome after ingestion
of pear and EAST to pear class 1 and higher (2)
-
mediator release (3, 5)
|
(1) Vieths et al. 2000
(2) Karamloo 1999a
(3) Scheurer et al. 1999
(4) Karamloo et al. 1999b
(5) Karamloo et al. 2000 |
Pyr c 4*
Expressed in
E.coli
Fusion peptide
-- |
immunoblot:
-
sera of 23 patients with pollen-related allergy to pear and
EAST to pear > 0.7 U/ml (1, 2)
immunoblot inhibition:
-
sera of 1 subject with oral allergy syndrome after ingestion
of pear and EAST to pear class 2 (2)
EAST:
-
sera of 61 patients with pollen-related allergy to pear (1,
2)
-
(reported, data not shown) sera of 16 patients with oral
allergy to pear (3)
-
(reported, data not shown) sera of 49 allergic subjects with
positive EAST to recombinant birch profilin (3)
EAST inhibition:
-
sera of 6 subjects with or without symptoms after ingestion
of pear but positive EAST to pear class 2 and higher (2)
mediator release:
-
sera of 5 patients with oral allergy syndrome after ingestion
of pear and EAST to pear class 1 and higher (2)
-
(reported, data not shown) (3, 4)
|
(1) Vieths et al. 2000
(2) Karamloo 1999a
(3) Scheurer et al. 2000a
(4) Karamloo et al. 2000 |
Pyr c 5*
note: in reference (1) denominated Pyr c 2
Expressed in
E.coli
Fusion peptide
-- |
immunoblot:
-
sera of 23 patients with pollen-related allergy to pear and
EAST to pear > 0.7 U/ml (1, 3)
immunoblot inhibition:
-
sera of 1 subject with EAST to pear class 2 (3)
EAST:
-
sera of 36 or 61 patients with pollen-related allergy to
pear (1, 2, 3)
EAST inhibition:
-
sera of 8 subjects with or without symptoms after ingestion
of pear but positive EAST to pear class 2 and higher (3)
mediator release:
-
sera of 5 patients with oral allergy syndrome after ingestion
of pear and EAST to pear class 3 (3)
-
(data reported, not shown) (4)
|
(1) Vieths et al. 2000
(2) Karamloo et al. 1999b
(3) Karamloo 1999a
(4) Karamloo et al. 2000 |
|
Atlantic Salmon (Salmo salar) |
Sal s 1*
Expressed in
E.coli
Fusion peptide
his10-tag |
immunoblot:
-
sera of 6 patients with clinical histories of allergy to
fish and cod fish-specific IgE of RAST class 3 and higher
ELISA:
-
sera of 4 patients with clinical histories of allergy to
fish and cod fish-specific IgE of RAST class 3 and higher
|
van Do et al. 1999 |
|
Mustard (Sinapis alba) |
Sin a 1*
Expressed in
E.coli
Fusion peptide
-- |
immunoblot:
-
pooled serum of 5 mustard-sensitive individuals
ELISA inhibition:
-
pooled serum of 5 mustard-sensitive individuals
|
González De La Peña et al.
1996 |
Sin a 1*
Expressed in
E.coli
Fusion peptide
glutathione S transferase |
immunoblot:
-
pooled serum of 5 mustard-sensitive individuals
|
González De La Peña et al. 1996 |
|
Wheat (Triticum aestivum) |
Wheat gliadins
Expressed in
E.coli
Fusion peptide
-- |
immuno dotblot:
-
sera of 10 patients with significant RAST against wheat
|
Maruyama et al. 1998 |
Wheat glutenins
Expressed in
E.coli
Fusion peptide
-- |
immuno dotblot:
-
sera of 10 patients with significant RAST against wheat
|
Maruyama et al. 1998 |
Wheat LTP
Expressed in
yeast: Pichia pastoris
Fusion peptide
-- |
RAST:
-
(reported, data not shown) sera of 34 patients with food
allergies, mainly against Rosaceae
|
Asero et al. 2000b |
|