Hel a 2 (Sunflower Profilin)

Allergen Data Collection: Sunflower Seed (Helianthus annuus)
Internet Symposium on Food Allergens 3(2):103-114 (2001) [http://www.food-allergens.de]

5.3 Properties of Sunflower Pollen Profilin (Hel a 2)

5.3.1 Molecular Biological Properties

Sunflower Profilin References

Allergen Nomenclature Hel a 2 (1) Allergen Nomenclature Sub-Committee 2001

Isoallergens and Variants
3 isoforms differing in pI (1) (1) Asturias et al. 1998

Molecular Mass 14.7 kDa (SDS-PAGE) (1) Asturias et al. 1998

Isoelectric Point pI 4.41, 4.46, and 4.50 (Agarose-IEF)
(1) Asturias et al. 1998

Amino Acid Sequence, mRNA, and cDNA

Proteins

SWISS-PROT:

GenBank: T31427 O81982 CAA75506

Amino Acids 133 (1) 133 (1) 133 (1)

mRNA precursor 402 bp (1)

cDNA precursor

(1) Asturias et al. 1998

recombinant Profilin
Expression in Escherichia coli:
rHel a 2 expressed as non-fusion form (1)
2 recombinant profilins as glutathione-S-transferase fusion-proteins (2) (1) Asturias et al. 1998

Biological Function
Binds to actin (1:1), affects structure of the cytoskeleton
profilin family (1) (1) SWISS-PROT

Sequence Homology
11 plant profilins: aa sequence identies 69% to 88% (1)
Bet v 2 from birch pollen: aa sequence identity 73% (1) (1) Rihs et al. 1999

5.3.2 Allergenic Properties

Sunflower Profilin References

Frequency of Sensitization
IgE-binding to profilin in 31% of patients (1) (1) see 6.1 Sensitization to Sunflower Allergens

Allergenicity of Recombinant Proteins
IgE binding capacity of rHel a 2 comparable to natural Hel a 2 (immunoblot inhibition, EAST inhibition) (1) (1) Asturias et al. 1998

Sunflower Profilin	References
*Frequency of Sensitization* IgE-binding to profilin in 31% of patients (1)	(1) see 6.1 Sensitization to Sunflower Allergens
*Allergenicity of Recombinant Proteins* IgE binding capacity of rHel a 2 comparable to natural Hel a 2 (immunoblot inhibition, EAST inhibition) (1)	(1) Asturias et al. 1998