Internet Symposium on Food Allergens 1(2):43-49 (1999)  http://www.food-allergens.de


Original:
Investigation of IgE-Binding Patterns and Allergenicity of Mango Fruit Allergens in Different Varieties

Heiko KINDER, Beate SCHARF, Hans STEINHART, Angelika PASCHKE

Institute of Biochemistry and Food Chemistry, University of Hamburg, Germany
 
 
SUMMARY
KEYWORDS
Mango has become one of the most popular tropical fruits. It is cultivated in more than 1000 strains. In the present study the four most frequently consumed varieties (Eden, Ngowe, Osteen and Tommy Atkins) cultivated in different continents were examined. In order to identify IgE binding allergens and determine mutual cross-reactivities of the varieties immunoblot and enzymeallergosorbent test (EAST) inhibition were performed. Seven sera from patients with sensitization to mango fruit were used. All four varieties shared at least five allergens with Mr of approximately 14, 30, 40, 43 and 67 kDa. Results of EAST inhibition elucidated almost the same inhibition curves and 50%-inhibition values for each variety. In conclusion it could be shown that there were no significant differences in allergenic potency among the four mango strains.
food allergy
mango fruit
varieties / strains
mango Eden
mango Osteen
mango Tommy Atkins
mango Ngowe
SDS-PAGE/immunoblot
immunoblot inhibition
EAST inhibition

[Introduction] [Material&Methods] [Results] [Discussion] [References] [Abbreveations]



Heiko Kinder
Correspondence:
Heiko Kinder 
Institute of Biochemistry and Food Chemistry
University of Hamburg
Grindelallee 117, D-20146 Hamburg, Germany

Phone: +49-40-42838-4268
Fax: +49-40-42838-4342
E-mail: kinder@lc.chemie.uni-hamburg.de


copyright © 1999 by matthias besler -  ONLINE PUBLISHER 
home: www.food-allergens.de