M. WIGOTZKI, S. SCHUBERT, H. STEINHART, A. PASCHKE
Institute of Biochemistry and Food Chemistry, University of Hamburg,
|The allergenicity of hazelnuts treated with different proteolytic enzymes was examined and compared by SDS-PAGE / immunoblotting and EAST- inhibition experiments using sera of 16 hazelnut allergic individuals. 30 min treatment with the enzymes protease, elastase and trypsin eliminated the allergenicity of the hazelnut proteins, which can be shown by means of immunoblotting experiments. EAST- inhibition experiments reveal only a reduction of allergenicity. Treatment with pancreatin led to a slight decrease in the IgE binding activity of the main hazelnut allergens after 30 min and to an elimination after 60 min. Hydrolysis with pepsin at pH 1.2 resulted in a slight decrease of allergenic activity after 60 min. A complete elimination did not even appear in two of seven patients' sera after 240 min of hydrolysis with pepsin. The results reveal that the IgE-binding proteins in hazelnuts are not very stable to in vitro digestion with proteolytic enzymes.
[Introduction] [Material & Methods] [Results] [Discussion] [References] [Abbreveations]
Dr. Angelika Paschke
Institute of Biochemistry and Food Chemistry
University of Hamburg
Grindelallee 117, D-20146 Hamburg, Germany