Internet Symposium on Food Allergens 1(4): 137-46 (1999)

The Major Allergen from Hen's Egg White: Ovomucoid (Gal d 1)

Matthias BESLER (a, c) and Yoshinori MINE (b)

(a) Institute of Biochemistry and Food Chemistry, University of Hamburg, Hamburg, Germany
(b) Department of Food Science, University of Guelph, Guelph, Ontario, Canada
(c) matthias besler ONLINE PUBLISHER, Hamburg, Germany
Ovomucoid (OM) is the dominant allergen from hen's egg white. Frequency of sensitization to OM varies from 34-97% in egg allergic patients. The carbohydrate moieties of OM present up to 22-29%. It consists of 186 amino acids forming three tandem domains, each providing trypsin inhibitory activity. Effective isolation and purification of OM was attained by combined IEC / SEC and by automated fraction collection using capillary zone electrophoresis. Matrix-assisted laser desorption / ionisation time of flight (MALDI-TOF) mass spectrometry revealed an average Mr of 28.0 kDa. Immunoblot and RAST inhibition experiments of native and deglycosylated OM indicate that only peptide epitopes participate in IgE- binding structures of OM. All 3 domains exhibit IgE- binding potencies. At least 8 independent IgE binding regions using synthetic peptides of OM were identified. T-cell proliferative response in PBMC from egg allergic patients could be induced by all 3 domains, predominantly by the second and third domains.
food allergy
egg white
posttranslational modifications
B-cell epitopes
T-cell proliferation
capillary electrophoresis

[Introduction] [Isolation & Purification] [Molecular Biological Properties] [Allergenic Properties] [Future Perspectives] [References] [Abbreveations]

Yoshinori Mine

Matthias Besler
Dr. Matthias Besler
matthias besler - ONLINE PUBLISHER
Mildestieg 17, D-22307 Hamburg, Germany
Phone: +49-40-6970-4097
Fax: +49-40-6970-4098

copyright © 1999 by matthias besler - ONLINE PUBLISHER